Completed on 22 Nov 2017 by Brian Grone. Sourced from https://www.biorxiv.org/content/early/2017/11/13/217810.
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It is not clear, from these data, whether the differences between these leopard and longfin zebrafish are due to inherent serotonin differences. The fluoxetine treatment did not have the predicted effect: instead, it “increased aggression in leopard animals and decreased it in longfin zebrafish.”
Separate experiments to specifically reduce and increase serotonin levels in each group would be necessary for making conclusions about the roles of serotonin in zebrafish aggression and strain differences.
Although previous studies may have found strain differences in zebrafish serotonin levels, such differences would need to be confirmed for the animals tested here before making inferences about the potential role of serotonin in their behavioral differences.
The figures could be presented more clearly. The individual data points are too small and there are an excessive number of white lines in the graphs. Data points can be jittered to avoid most of the overlap.
Line 47 – Extant species are not “basal”. This is a common misconception.
Line 92 – Common leopard and longfin zebrafish strains are inbred, though not as inbred as some other model organisms. Actually, “leopard” and “longfin” just describe one aspect of the phenotype (pigmentation or tail morphology), so it is not clear from this description what strains were used. Obtaining fish from ZIRC or another lab could help identify the strain, as could some genomic data.
Line 146-148 – Why was this statistical approach chosen? What does a standard t-test show?
Line 166 – The word “injected” is duplicated.