Review for "A portable system for metagenomic analyses using nanopore-based sequencer and laptop computers can realize rapid on-site determination of bacterial compositions"

Completed on 21 Jan 2017 by Keith Robison . Sourced from http://biorxiv.org/content/early/2017/01/20/101865.

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Comments to author

Figure 4b: please make ines much heavier & cinder avoiding color for distinguishing stages - different shapes would work better (e.g. rectangle, hexagon, oval) for individuals with color perception issues.

If you are going to claim portability, then a complete list of equipment is needed, with weights. What are power requirements? Refrigeration requirements?

What is the yield from each run? What fraction of reads in each were unclassifiable? What is expected sensitivity in a more complex sample?

Discussion and legend for 4b should emphasize that you started with purified DNA, not bacteria. So time to lyse&purify DBA would need to be added to running time, and differential lysis/extraction could shift your sensitivity.